Cloning and expression of Neospora caninum dense-granule 7 in E. coli
نویسندگان
چکیده
منابع مشابه
First Characterization of the Neospora caninum Dense Granule Protein GRA9
The obligate intracellular apicomplexan parasite Neospora caninum (N. caninum) is closely related to Toxoplasma gondii (T. gondii). The dense granules, which are present in all apicomplexan parasites, are important secretory organelles. Dense granule (GRA) proteins are released into the parasitophorous vacuole (PV) following host cell invasion and are known to play important roles in the mainte...
متن کاملGRA 14, a novel dense granule protein from Neospora caninum.
Dense granule protein (GRA) is a secreted protein in Neospora caninum and Toxoplasma gondii, which plays an important role in forming parasitophorous vacuole (PV) [1–4]. GRAs are thought to remodel and maintain the environment of the PV for intracellular survival and replication in T. gondii [5]. The precise function of GRAs is still unknown [6–9]. TgGRA14 protein is a new GRA protein that it i...
متن کاملImmunization with oligomannose-coated liposome-entrapped dense granule protein 7 protects dams and offspring from Neospora caninum infection in mice.
The present study demonstrates that the subcutaneous administration of Neospora caninum dense granule protein 7 (NcGRA7) entrapped in liposomes coated with mannotriose strongly induces the parasite-specific T-helper type 1 immune response and humoral antibody in mice. Although anti-NcGRA7 immunoglobulin G1 antibody production was induced in mice injected with NcGRA7 alone, the dams and offsprin...
متن کاملCloning and expression of rhl AB operon under the control of tac promoter in E. coli
Today, efforts go towards the replacement of chemical surfactants by natural biological biosurfactants (biosurfactant), as these materials are not carcinogenic and highly compatibile with the environment. One of the main classes of biosurfactants is rhamnose containing glycolipid biosurfactant (rhamnolipids). This type of biosurfactants can be applied in many industries such as oil industry, ph...
متن کاملCLONING AND EXPRESSION OF A HUMAN INTERFERON a2 GENE IN E. COLI
The plasmid pALCA1SIFN containing cDNA that encodes the human interferon a-2b was obtained from the ATCC(no. 531667). In this system the expression of the gene is under the control of an alcA promoter. alcA p is a specific promoter for expression of different genes in Aspergillusfilamentous. In this plasmid the coding region of IFN?-2b is preceded by the coding region of a synthetic signal ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Parasitic Diseases
سال: 2012
ISSN: 0971-7196,0975-0703
DOI: 10.1007/s12639-012-0221-1